FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44853279
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1467310.3271355032928585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT273950.025.68120845
AGACCGC300550.023.492234
CAGACCG309200.022.8859963
AGAACGA318250.022.17018544
GACCGCG320800.022.0023085
ACCGCGT332700.021.315796
CCGCGTT342700.020.7132767
CGCGTTC348750.020.3475028
GCGTTCT359450.019.772899
AAGAACG357150.019.7318943
CTCAGAC384450.019.5129571
TCAGACC369700.019.4816132
TACGGAG369200.019.09560634
ATACGGA371300.019.00580233
AATACGG371900.018.94980232
CAATACG383800.018.4966431
CCGTACG64400.018.3717084
CGTACGC65150.018.0221145
ACGGAGA394950.017.90824535
CCCAATA404700.017.62914329
Produced by FastQC (version 0.11.5)