FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45968132
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1407070.30609684117683966No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1051060.22864970888962816No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT462050.10051528741694354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG38100.023.5590026
CGGTATA57000.021.94947620
AGACCGC321650.021.4270154
CAGACCG330150.020.9435123
GACCGCG339050.020.2529965
ACCGCGT344200.019.9864466
GAACGAT354350.019.43988445
CCGCGTT357050.019.2544617
TCGACGA48500.019.06377
CGCGTTC360500.019.0514768
GTTCGAC46800.018.9416965
TCAGACC390750.018.0639952
GCGTTCT384500.017.9149639
GCCGGCT307500.017.5712281
CCGGCTT350200.017.463512
CTCAGAC431650.017.362731
CGACGAG53000.017.2753018
TCACGCT352900.016.8672878
TTCACGC357550.016.7108447
CCGTACG60900.016.330644
Produced by FastQC (version 0.11.5)