FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45895566
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA119200.018.87525223
ACGCTAC121750.018.47991824
CGCTACC124750.017.96329125
AGACCGC113000.017.742014
GACCGCG118000.017.0092985
ACCGCGT123150.016.316496
AAAACGC139000.016.28366721
GTCCGTT35900.016.180731
TTCACGC148050.016.1550777
GAACGAT126050.015.99658645
TTCGGCG50450.015.96516244
CCGTACG49800.015.9495684
CGTACGC50650.015.7707545
ACGCTCA155200.015.69979310
CGCGTTC129800.015.5306828
TCACGCT153350.015.4490488
AAACGCT146950.015.37209622
GAAAACG146250.015.35328420
CCGCGTT131500.015.2966367
CAGACCG132400.015.2443173
Produced by FastQC (version 0.11.5)