FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45895566
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG18600.013.3059326
AGCTTAG127900.012.98253145
ATAGGGG131600.012.87419838
GGCATAG141050.011.82076635
GGGAGGT218100.011.5973173
GAGCTTA156950.010.66559744
GGAGGTC234450.010.5676364
CATAGGG162500.010.52304337
GCATTCG39600.010.34244253
GTCACTC242850.010.3207828
GGTCACT238950.010.15967757
TGGCATA164450.010.1525134
TCGCGCT37950.09.78240645
CGCTCGC21750.09.72374625
GCATAGG178100.09.63921636
ACTCTCC258950.09.4097511
GTTCTAT114250.09.2970061
CCTAGGC84500.09.2659098
TATCCTA91050.09.2181865
ACCTAGC41850.09.1395266
Produced by FastQC (version 0.11.5)