FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56995981
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG37750.021.6333226
AGACCGC95150.019.0121274
CAGACCG100750.018.3573593
CGGTATA60600.018.2755620
CGTTACG18850.017.30566421
CGACGAG46900.016.9311568
TCGACGA51050.016.7462927
GTTCGAC48550.016.5911795
GACCGCG109700.016.428935
GTCCGTT45450.016.1881521
CCCGTAT45000.015.3500321
CTCCGAT59200.015.2027341
CCGTACG46650.015.1447274
ACCGCGT119450.015.1427036
GAACGAT120350.015.12241245
CCCGGAT138000.015.0978581
CCGCGTT122600.014.7535467
CGCGTTC124900.014.4983378
CGTACGC48900.014.4938965
GCACTAC80050.014.44399325
Produced by FastQC (version 0.11.5)