FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56995981
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG28150.012.94811826
ATAGGGG147750.011.83176338
AGCTTAG155850.011.08750945
GGCATAG153750.011.01883835
GGGAGGT236600.010.43421653
CATAGGG174550.09.796020537
GGTCACT235800.09.5627747
GAGCTTA179200.09.54203644
TGGCATA182550.09.44067134
GTCACTC243600.09.43218
GCATAGG182700.09.40829136
ACTCTCC249750.09.38706811
GGAGGTC255300.09.3790484
GAGGTCA272250.08.9604245
AGGTCAC276450.08.7753036
TGTCGTT45150.08.62087228
TAATCGG44900.08.620854
CACTCTC275900.08.34275710
TCACTCT285650.08.28679
AACTCTA131300.08.25965445
Produced by FastQC (version 0.11.5)