FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49873405
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1946750.39033829753553023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT340500.027.29654545
ACCGCGT356950.026.0907426
AGACCGC362050.025.890994
GACCGCG361350.025.797985
CAGACCG369550.025.4690173
CCGCGTT373050.024.9466297
CGCGTTC376600.024.7503918
AGAACGA393650.023.59972244
TCAGACC409900.023.2918472
GCGTTCT434650.021.51739
AAGAACG435950.021.30469343
AATACGG438350.021.18488932
ATACGGA438900.021.127233
CTCAGAC475950.021.020691
TACGGAG440150.020.97890534
CAATACG452050.020.66750131
ACGGAGA459100.020.14705535
ACTCCCC470400.020.08590125
CGGAGAG470950.019.53023536
CGTTCTC498450.019.0926510
Produced by FastQC (version 0.11.5)