FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45012277
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT610310.13558745317416404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT135000.021.59894845
TTCGACG16800.018.2133966
AGACCGC168950.017.8325984
AGAACGA179700.016.9774744
CAGACCG183700.016.6334883
GTCCGTT35050.016.5631241
GACCGCG183450.016.4230985
ACCGCGT188700.016.1677326
TTCGGCG86950.015.96529344
CCGCGTT193500.015.6969097
CGCGTTC195150.015.5987788
TCGGCGC89200.015.33557345
GCCGGCT172100.015.2842511
AATACGG200650.015.13756232
ATACGGA201750.015.03272333
CCGGCTT196400.015.0194452
AACGCTA164600.014.90124723
TACGGAG204100.014.85963734
CTACACG75200.014.74994545
ACGCTAC167000.014.66014924
Produced by FastQC (version 0.11.5)