FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45012277
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG496170.11022992682640782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG168100.013.61436445
GGGAGGT263800.012.40877153
GTTGTCG21200.012.20253926
ATAGGGG187450.012.13496138
GGCATAG186850.011.94427835
TCAGACG88650.011.7330432
GAGCTTA200650.011.36094544
GGAGGTC286150.011.2743414
ACTCTCC292300.010.92337111
TGGCATA206050.010.89583934
CATAGGG217200.010.36903437
ATAGCTC55400.010.0719439
GCATAGG226950.09.95339936
CAGACGT102350.09.9423673
GTCACTC327250.09.7431658
GGTCACT328800.09.6148037
ACCCGGC65400.09.5009415
TGGGAGG258700.09.4771742
CACTCTC338750.09.3326410
GCTCTGT345350.09.22986322
Produced by FastQC (version 0.11.5)