FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53272950
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG901260.16917779098022542No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT785530.14745382037225271No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG634290.11906417797399994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC168000.024.386674
TTCGACG23150.024.1991546
CAGACCG178250.023.0348383
GACCGCG200100.020.5420275
TCGACGA28500.020.0512147
ACCGCGT216650.019.0039656
CGGTATA39750.018.50996820
CGACGAG30550.018.4848698
GTTCGAC30650.018.3510685
GCGTTCT224800.018.2049839
GCCGGCT274800.018.147491
CCGCGTT227550.018.0936457
CCGGCTT315150.017.8331222
TCAGACC241700.017.760452
CGCGTTC232950.017.626018
CTCAGAC288700.017.2269121
GAACGAT245850.016.55465745
TCACGCT330800.016.2753778
CGTTACG20500.016.2451521
TTCACGC332900.016.233467
Produced by FastQC (version 0.11.5)