FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53272950
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG823870.1546507186104768No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA642680.1206390860652545No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG599830.11259560433578393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT315400.016.6902683
GTTGTCG32400.015.47963426
GGAGGTC347750.014.9926074
TGGGAGG312050.013.4811312
AGCTTAG300100.013.46343945
ATAGGGG302150.013.347712538
GGCATAG300700.013.23933635
ATATAGC45350.012.952086
GAGCTTA320750.012.48502944
GGTCACT407800.012.4335637
ACTCTCC425350.012.02716311
TGGCATA337200.011.90637334
GTCACTC428250.011.8143868
CTGGGAG404850.011.6984791
AGGGATC200350.011.6676032
TGTCGTT46050.011.38227628
TCAGACG93300.011.1997912
CAGGGAT240650.010.6872131
GCTAAGT50150.010.59038314
CACTCTC484300.010.56822710
Produced by FastQC (version 0.11.5)