FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48379152
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG494790.1022733924728569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG174950.014.45548345
ATAGGGG185200.013.65542338
GGCATAG187700.013.37764635
GGGAGGT288200.013.358453
GAGCTTA199750.012.67201144
GGAGGTC307500.012.1102444
TGGCATA209950.012.08851734
CATAGGG212750.011.86596637
GGTCACT330950.011.4147967
GTCACTC326650.011.4135228
ACTCTCC333950.011.15728911
GCATAGG230250.011.02273236
TAGGGGA347700.010.386044539
TGGGAGG276300.010.1225582
TCTCCCC367850.010.10459913
AGCATAG109900.010.03179545
GCTCTGT379000.09.87264122
TCTGTCC380400.09.81856124
CGTAGGG110950.09.75433337
CTCTGTC388350.09.68129423
Produced by FastQC (version 0.11.5)