FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51635879
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2110280.4086848216527892No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1054710.2042591353969204No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA951630.18429627197786252No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC625550.12114638350593393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT413200.025.04814545
AGAACGA533400.019.4163944
AGACCGC608450.016.9642374
ACCGCGT605700.016.9213316
GACCGCG608200.016.8484065
CAGACCG613600.016.8255523
CGCGTTC618550.016.5763078
CCGCGTT618550.016.5696287
TCGGCGC70500.016.03101745
TCAGACC665050.015.8351212
GCGTTCT659950.015.5637119
AAGAACG670300.015.45755743
CTCAGAC717300.015.3723221
CGTACGC75950.015.3136835
CCGTACG75750.015.2656834
CGTTACG20650.015.14940121
TTCGACG18550.015.0378676
AATACGG710650.014.49733432
CGTTCTC727550.014.45765410
ATACGGA716850.014.37356733
Produced by FastQC (version 0.11.5)