FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46095831
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG541360.1174422910392916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG180050.014.73365445
ATAGGGG194550.013.69302738
GGTCACT272900.013.6203217
GGCATAG197400.013.42697135
GGGAGGT292700.013.3986833
GTCACTC282750.013.0582768
GAGGTCA307900.012.7006975
ACTCTCC298750.012.441874511
GGAGGTC313850.012.2089974
GAGCTTA218350.012.18012444
TGGCATA219150.012.14571834
AGGTCAC323050.011.9934536
GTTGTCG22100.011.80989626
TCACTCT329250.011.54210959
CACTCTC329500.011.27393110
CATAGGG241050.011.11689837
GCATAGG247500.010.81811636
TGGGAGG275950.010.3960232
GCTCTGT376200.09.93418222
TCAGACG80750.09.8360252
Produced by FastQC (version 0.11.5)