FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40496182
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG571830.1412059042998177No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT263750.015.8259583
GGAGGTC281250.014.6321844
ATAGGGG212400.013.89743238
GTTGTCG21550.013.57407726
GGCATAG222400.013.35360935
AGCTTAG219100.013.34883445
TGGGAGG249350.012.6496662
GGTCACT325350.012.3362217
TGGCATA242900.012.24513234
GAGCTTA241350.012.13691244
ACTCTCC341600.011.75608511
GTCACTC348250.011.666868
CTGGGAG306750.011.2441511
GAGGTCA378350.011.1024335
CACTCTC367050.010.97764110
AGGTCAC391600.010.6974546
ATATAGC34850.010.3290586
CATAGGG289850.010.32368437
TCACTCT404950.010.2390429
TCTCCCC397200.010.12219913
Produced by FastQC (version 0.11.5)