FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47640067
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT992930.20842330049619787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG29300.023.9560386
AGACCGC217150.023.1554374
CAGACCG224800.022.5001283
GACCGCG231400.021.6904375
ACCGCGT241200.020.9302086
CCGCGTT242500.020.8272827
CGCGTTC245300.020.5987198
GAACGAT244450.020.27443745
GTTCGAC36850.019.41435
CGGTATA46150.018.86644420
TCAGACC273400.018.8458232
CTCAGAC312350.018.0844361
CGACGAG39000.017.997748
GCGTTCT283700.017.9541829
TCGACGA42100.017.1534297
TACGGAG295800.016.69890234
AATACGG299800.016.55929432
ATACGGA300500.016.4677133
AGAACGA303300.016.3553644
CAATACG317100.015.79117631
Produced by FastQC (version 0.11.5)