FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47640067
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG27500.014.07269926
ATAGGGG117200.011.11468138
TAATCGG54300.010.6077914
GTAACGA53100.010.38158618
AGCTTAG129350.010.24507145
GGCATAG124750.010.24347935
ATAATCG60600.010.1398993
ATCGGTT63600.010.0108376
TGTCGTT39650.09.53341828
GGGAGGT192700.09.4728963
ACACTCG105800.09.4211274
CACTCGA101750.09.3535345
AATCGGT63850.09.3380185
GTCACTC185800.09.2989078
GGTCACT186050.09.1531927
CATAGGG142350.09.08792337
ATATAGC61450.09.0430756
CGTAACG13100.08.93078242
AGGGATC135850.08.8839812
GGTAACG62400.08.83454417
Produced by FastQC (version 0.11.5)