FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43127811
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT735130.17045381691178343No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG696490.16149440090989084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT158750.023.8791086
GACCGCG158600.023.7316655
AGACCGC163650.023.2061714
CGCGTTC169300.022.3245268
CCGCGTT169800.022.2985367
CAGACCG174600.021.8926163
GCCGGCT185300.019.8003081
CCGGCTT223450.019.0410522
TCAGACC210550.018.850512
GCGTTCT208800.018.2628759
TCACGCT240350.017.6252828
TTCACGC239500.017.612697
CTCAGAC272650.016.618241
AACGCTA244700.016.61660223
ACGCTAC245750.016.54560724
GCACTAC74100.016.30762725
CGGCTTC276000.016.1889513
CGCTACC252050.016.1058825
ACGCTCA267600.016.0658810
CACGCTC270300.015.6723529
Produced by FastQC (version 0.11.5)