FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43127811
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG157700.013.33960638
GGGAGGT225350.013.1502983
GGCATAG158200.012.89942835
GGAGGTC219750.012.8607754
AGCTTAG159000.012.81989945
GTTGTCG24300.012.77913226
TGGCATA178500.011.47024834
GAGCTTA178600.011.45065844
ACTCTCC267300.010.79018711
GGTCACT258850.010.7421727
GTCACTC263250.010.5628348
CATAGGG200450.010.29270237
AGGGATC148050.010.18716052
GCATAGG209950.09.81626336
GAGGTCA297750.09.7781945
TGTCGTT34150.09.55445428
CACTCTC297350.09.46512310
TCGCGCT20350.09.28684945
TAATCGG51550.09.1663634
AGGTCAC321150.09.120626
Produced by FastQC (version 0.11.5)