FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47950549
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1139740.2376907092346325No Hit
CCCCACGATGCTGTCCAGCTGCCTCCTGAGGTTGTTGATGTACTGCTCGAA619450.12918517366714613No Hit
CTCCAGGTTCTGCCTCACAGTCTTGGTGCCCTGCTCCTGCAGCAGGGTCCA590700.12318941332663366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT282800.021.53308545
AGACCGC291950.020.8020384
ACCGCGT290600.020.7775236
CAGACCG297550.020.5088443
GACCGCG295450.020.4245415
CGCGTTC298300.020.2560888
CCGCGTT299300.020.1959257
ACGTTCG16450.018.7353386
TCAGACC331850.018.6399362
AGAACGA327700.018.61040744
CTCAGAC386200.016.9588431
GCGTTCT365800.016.7027849
CCGTACG78250.016.0745544
AATACGG382350.015.97061932
TACGGAG385800.015.74490734
ATACGGA387800.015.71626433
AAGAACG391350.015.64105343
CAATACG401900.015.250236531
CGTACGC84100.015.1157595
CCCAATA387450.014.88678829
Produced by FastQC (version 0.11.5)