FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47950549
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG485270.10120217810227783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG160550.014.33096845
GGCATAG173650.013.30676135
GGGAGGT308150.013.0556343
GTCACTC284300.012.9404228
ATAGGGG183800.012.63306138
GAGCTTA191350.012.14093344
TGGCATA192800.012.00869834
ACCCGGC83450.011.7021215
GGAGGTC338800.011.4359774
AGCATAG127300.011.08853245
TCACTCT341400.010.9342639
CATAGGG215350.010.85512237
CACACTC219300.010.75221624
TCACCCG96600.010.6213643
TCACACT232500.010.45057823
ATAGCTC85850.010.352468539
CCCTCTC231150.010.3277334
TCGCTCG45550.010.32364924
ACGGACA88600.010.25700318
GGTCACT376550.010.2479887
Produced by FastQC (version 0.11.5)