FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45601569
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT38100.020.6096311
TCGTGTA38550.020.31075514
GTCGTGT42350.018.59455713
TGTAGGG52900.015.82241417
GTAGGGA52550.015.28554618
ACCCGGC77300.014.5823665
AGCGTCG58950.014.00722310
GGTCGCC58350.013.92119843
AGTGTAG61750.013.77287728
ATCTCGG61300.013.65470335
GATCGGA61200.013.3819751
TCTCGGT65950.012.99937236
GTCGCCG70900.012.78995744
AGAGCGT69300.012.1425018
TCACCCG100550.012.0384343
GTAGATC69450.011.95429331
GTGTAGG69450.011.8575216
GATCTCG70150.011.7708634
GTTGTCG18500.011.432096526
CGGCCCG103100.011.2824048
Produced by FastQC (version 0.11.5)