FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44935821
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT519570.11562490423842485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC119300.022.5934964
CAGACCG126800.021.5232893
GACCGCG130300.020.5993465
ACCGCGT137300.019.6792556
CCGCGTT146800.018.420797
CGCGTTC148000.018.210638
AACGCTA174150.016.55127123
ACGCTAC177750.016.28009424
CCGGCTT195200.016.1145112
GCCGGCT176850.016.080891
CGCTACC181950.015.79327225
CCCGTAT32650.015.5417331
CTCAGAC210700.015.4776091
TCACGCT199650.015.4151118
GAACGAT172700.015.41062745
GCGTTCT175650.015.3824059
TCAGACC183100.015.323852
AAAACGC192750.015.01202421
TTCACGC204350.015.0055217
CCGTACG24950.014.9694064
Produced by FastQC (version 0.11.5)