FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48147917
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3114190.6467964128126249No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1079670.22424023037175214No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1056620.21945289969657464No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC639630.13284686853638963No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC555610.11539647706877952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT494200.030.43424845
AGAACGA620450.024.28496244
ACCGCGT706650.021.3701656
GACCGCG709450.021.2696485
AGACCGC713600.021.1648484
CCGCGTT718250.021.0249867
CGCGTTC715700.020.9269988
CAGACCG724500.020.902333
AAGAACG746750.020.18669943
TCAGACC755800.020.1647422
GCGTTCT761000.019.772939
CTCAGAC813500.019.4494171
AATACGG782650.019.27315932
ATACGGA786150.019.18753233
TACGGAG793950.018.96279734
CAATACG811950.018.6275731
ACGGAGA815900.018.4471135
CGGAGAG822950.018.250836
TCGGCGC104100.018.09000245
CCCAATA841650.018.03372829
Produced by FastQC (version 0.11.5)