FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48147917
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG710910.14765124730110338No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA664840.13808281674989178No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG655750.13619488460944218No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG548010.11381800795245202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG241150.014.68207945
GGCATAG257450.013.62799635
GGTCACT372400.013.3610647
GGGAGGT393050.013.3370663
GTCACTC377650.013.2468948
ATAGGGG273050.013.03869238
TGGCATA273450.012.9045134
GAGCTTA277200.012.85386744
GGAGGTC401750.012.7979354
GTTGTCG25150.012.61512926
ACTCTCC403550.012.37611811
CACTCTC408650.012.30394710
TCACTCT438750.011.7412839
CATAGGG302900.011.7166937
AGGTCAC442850.011.5500676
GCATAGG320000.011.06968836
GAGGTCA471500.010.9146695
TATCCTA99700.010.806295
TGCCCGT126400.010.73782544
AGGGATC171250.010.6891112
Produced by FastQC (version 0.11.5)