FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45316848
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1292000.28510367711364215No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT897370.19802127455996058No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG825790.18222582470872642No Hit
GGGGAAACAGAGTCGTGGAGGCTTTGAATCTCTCAGAAAAAAGGAAAGACA567700.1252734965150268No Hit
GGGGGAAACAGAGTCGTGGAGGCTTTGAATCTCTCAGAAAAAAGGAAAGAC464980.10260643017360783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT323550.020.8290671
CCGGCTT370250.020.6813032
CGGCTTC409000.019.211533
TTCACGC393950.019.0640247
TCACGCT393600.019.0123838
AGACCGC264600.017.2375264
ACGCTCA449850.017.14519510
GACCGCG266250.016.9781135
CAGACCG273500.016.874053
ACCGCGT268100.016.8262356
CACGCTC451700.016.7661519
GCACTAC101650.015.93762925
GTTCCTA75700.015.66341615
GTCCGTT36150.015.6235541
CCCAGAT195200.015.5966881
CCGCGTT293150.015.3884137
CGCGTTC296600.015.2245898
GGCTTCA545900.014.97075754
TCAGACC313750.014.902972
CCCGTAT33750.014.4010561
Produced by FastQC (version 0.11.5)