FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45316848
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG894460.19737912928101267No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA797210.17591911952923117No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG617990.13637091441134652No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG537050.11851000758040366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG52250.017.43496926
ATATAGC62050.015.9946256
GGGAGGT296350.014.5562053
GGAGGTC296450.014.3839434
TGTCGTT67700.013.8881828
AGCTTAG233350.013.82913945
TATAGCT71550.013.8066117
GCTAAGT66950.013.71408214
AGGGATC208050.013.2891112
ATAGGGG249250.013.14245338
TCGTTCC70350.013.04511930
GGCATAG249700.012.94597535
CAATATA85200.012.7366994
GAGGTCA337050.012.7310615
GTCGTTC75500.012.66160229
GAGCTTA266800.012.23870344
TGGCATA266150.012.12866234
CGTTCCC75400.012.11174431
AATATAG92550.012.0897135
CAGGGAT247300.012.0086481
Produced by FastQC (version 0.11.5)