FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50217129
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT590620.11761325503096762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC129400.023.474214
GACCGCG132700.022.8056055
ACCGCGT133450.022.6434426
CAGACCG139700.021.9208133
CCGCGTT143650.021.0039427
CGCGTTC144250.020.4326618
TTCGGCG94400.017.18431944
TCAGACC184700.016.946782
TCGGCGC95650.016.8187645
CCGTACG101050.016.4773334
CGTACGC104300.016.0285665
TACGCCA109550.015.3626787
CTCAGAC238200.015.3371531
CTCCGGT62800.015.1258851
GCGTTCT198200.014.9952579
TCCGTAC112900.014.9472853
GAACGAT202450.014.82555545
ACGTTCG16300.014.6319866
CGCCCCG114100.014.61175723
CGCGGGG110650.014.51908231
Produced by FastQC (version 0.11.5)