FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50217129
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC106200.015.9322365
TCACCCG117300.014.8021033
GGCATAG174350.013.61465335
AGCTTAG178500.013.31870745
GTCACTC263900.013.3130958
CGGACAG129300.012.82301119
CGGACAC130950.012.80394413
CACCCGG136950.012.6504274
CCGGACA137050.012.43131812
GGGAGGT332500.012.4121943
TGGCATA194050.012.302217534
ATAGGGG202550.012.19602838
GCCCGGA142050.012.16828610
GAGCTTA196450.012.15984144
ACGGACA137500.012.05811318
TAGCGTA9900.012.04527437
GGTCACT297050.012.0088137
ACACGGA136850.012.00054216
TCACTCT304100.011.8862439
ATAGCTC134000.011.86981339
Produced by FastQC (version 0.11.5)