FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43500439
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG735710.16912702880998512No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG501390.11526090575775569No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT457050.10506790517677304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG35150.024.578896
AGACCGC109800.022.2322674
CGGTATA50200.021.82845520
CAGACCG115300.021.3668843
GTTCGAC40000.021.092485
TCGACGA41300.020.6464567
GACCGCG120250.020.2253935
CGACGAG42600.019.4882628
ACCGCGT126450.019.3760576
CCGCGTT133300.018.3803637
CGCGTTC134200.018.2738638
GCCGGCT231850.017.6787431
CCGGCTT275650.017.376612
CCGTACG47050.016.8321424
AGTTCGA52500.016.2847354
CGTACGC49850.016.2477685
GCGTTCT157000.015.7490519
TCACGCT299150.015.61338
TTCACGC299950.015.5491537
CTCAGAC204700.015.532271
Produced by FastQC (version 0.11.5)