FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41450224
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC67500.017.1340644
ACCGCGT70150.016.32296
GACCGCG73950.015.5167245
GTCCGTT36850.015.3955881
CCGTACG38500.014.9032334
CAGACCG77800.014.8656733
CGTTACG15300.014.70869221
CCGCGTT78650.014.6159787
CGTACGC41050.014.3600565
AACGCTA92100.014.27068923
ACGCTAC95300.013.88680524
CGCGTTC83050.013.5165728
CCCCGCA176400.013.44179135
CGCTACC98100.013.32984325
ACGTTCG12350.013.297296
AAGCGGC180400.013.1493328
GAACGAT88450.012.80635545
TACGCCA47250.012.5692047
GTCCGGT32350.012.4569571
CCCGTAT27900.012.4265271
Produced by FastQC (version 0.11.5)