FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41450224
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT223800.012.7384243
AGCTTAG144450.012.49882445
ATAGGGG149900.011.72285438
GGAGGTC242200.011.5847814
GGCATAG151900.011.50917135
GTTGTCG21900.011.30217326
GAGCTTA165450.010.88444244
GGTCACT255600.010.6604917
GTCACTC263050.010.4442458
TGGCATA170350.010.36861934
ATATAGC32100.010.1635726
TGGGAGG205100.09.42367652
CATAGGG195600.09.13324537
CACACTC155200.08.93033424
TGTCGTT31150.08.88516828
TCAGACG60850.08.8005382
AGGGGAG313650.08.70890740
TAGGGGA309850.08.692348539
TCACTCT325100.08.6030479
TCCCTCT180850.08.5972333
Produced by FastQC (version 0.11.5)