FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43653661
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2080740.47664730800012395No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG615520.14100077425350419No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA549330.1258382429826447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT326750.029.99955745
AGAACGA395200.024.8209844
AGACCGC438050.022.521014
GACCGCG441750.022.3170995
CAGACCG444000.022.2900183
ACCGCGT443850.022.246996
CCGCGTT455100.021.7019927
AAGAACG458450.021.37201943
CGCGTTC472000.020.9630838
TCAGACC485150.020.4040362
AATACGG483000.020.35703532
TACGGAG490800.020.02406734
ATACGGA492100.019.98516333
CAATACG506000.019.49832531
GCGTTCT517700.019.129959
CTCAGAC538800.019.0469321
CGGAGAG516250.018.98460436
CCCAATA523550.018.89225229
ACGGAGA526600.018.67558535
ACTCCCC537950.018.45341925
Produced by FastQC (version 0.11.5)