FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49669875
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG159050.013.3863945
ATAGGGG163550.013.20825338
GGCATAG164350.012.91104535
GTTGTCG18650.012.06419126
GGGAGGT256100.011.7367683
GGTCACT249300.011.5778657
TGGCATA185050.011.52750134
GTCACTC255800.011.3364348
GAGCTTA191150.011.24427944
CATAGGG193050.011.20156537
ACTCTCC268900.010.93459611
GGAGGTC278350.010.7014424
GCATAGG207600.010.45979936
GAGGTCA296950.010.1978175
TCACTCT301700.09.8651239
CACTCTC300250.09.77789710
TAGGGGA305650.09.31316739
AGGTCAC338550.09.0439766
GCTCTGT328900.08.74920122
ACACTCG108450.08.712954
Produced by FastQC (version 0.11.5)