FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48328033
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA624770.1292769353968948No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG606290.12545306778779927No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG592800.12266172720085669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG190500.014.91749445
GGCATAG209400.013.74273835
GGTCACT308350.013.4448317
ATAGGGG218550.013.29084438
GGGAGGT342800.013.0405093
GTCACTC322900.013.0135258
GAGCTTA221250.012.97650144
GTTGTCG21450.012.90197926
ACTCTCC339700.012.45677111
AGGTCAC347950.012.2666986
TGGCATA237650.012.20379634
TCACTCT362750.011.6895849
GGAGGTC370850.011.6778374
GAGGTCA371550.011.6436725
CACTCTC362600.011.58903710
CATAGGG254500.011.4221537
GCATAGG265450.010.9340636
AGGGATC181450.010.54439452
TGGGAGG300550.010.3128082
ACCCGGC80750.010.2579175
Produced by FastQC (version 0.11.5)