FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51404371
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT4000170.7781770153359138No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1137850.22135277173219373No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG961600.1870658041900756No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC598330.11639671653603154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT707000.027.89012145
AGACCGC837100.023.7265474
CAGACCG840750.023.6826653
AGAACGA846950.023.31875644
GACCGCG856600.023.1285765
ACCGCGT864900.022.9794586
CCGCGTT871450.022.8041577
CGCGTTC875000.022.7347818
TCAGACC887100.022.7268122
CTCAGAC942150.021.9572721
GCGTTCT909850.021.8862239
AAGAACG957950.020.67763543
CGTTCTC987350.020.4189610
AATACGG1010300.019.49137332
ATACGGA1011400.019.463533
TACGGAG1008600.019.45726234
CCTCTCA1043050.019.35113319
ACTCCCC1033050.019.31211525
CAATACG1030950.019.18389331
ACGGAGA1038350.018.89255335
Produced by FastQC (version 0.11.5)