FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51404371
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG672660.13085657637946782No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT633140.12316851421059116No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA589940.11476455961303368No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG560230.10898489546735239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG26300.014.02872426
AGCTTAG215800.013.45740445
GGGAGGT324700.013.2736143
ATAGGGG229000.012.79214138
GGCATAG228550.012.71765735
GGAGGTC349900.012.26468854
GGTCACT352950.011.7637937
GAGCTTA251600.011.6248744
TGGCATA251900.011.55570434
ACTCTCC370650.011.37770111
GTCACTC372050.011.3534648
TGTCGTT36150.010.70275628
CACTCTC393350.010.63528610
CATAGGG286400.010.40860237
TGGGAGG304750.010.2953222
GCATAGG296300.010.09098436
GAGGTCA437000.09.9902325
TCACTCT434550.09.9586369
AGGTCAC439900.09.8632066
GTCGTTC40450.09.5648729
Produced by FastQC (version 0.11.5)