FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45410063
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG713870.15720524325191973No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT534110.1176193038974643No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG520190.11455390405426216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG52800.028.7960826
GTTCGAC56400.026.5204495
CGACGAG60600.024.384268
TCGACGA63900.023.93457
CGGTATA76750.023.853820
AGTTCGA74900.020.5405774
AGACCGC133600.019.5462784
GACCGCG132600.019.5070975
ACCGCGT137700.018.849186
ATGACGG94650.018.72477716
CAGACCG141250.018.662893
AACGACG25700.018.640516
GACGGTA103700.017.979918
GACGAGG103450.017.9146279
GCCGGCT216800.017.767931
ATAGAGT121100.017.64375324
GTATAGA123150.017.514422
ACGAGGA106700.017.4111210
CCGGCTT269300.017.3643632
CAGTTCG92500.017.2889123
Produced by FastQC (version 0.11.5)