FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44719529
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG527690.11799990111702652No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT516150.11541937304393345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC110550.023.0819874
CAGACCG118750.021.582863
GACCGCG140350.018.1810725
CCCGTAT27250.016.8373151
ACCGCGT156300.016.478656
CCGTACG49250.015.8998084
GCCGGCT182400.015.8896311
CGTTACG16000.015.88673121
CCGGCTT207600.015.62151052
CGAACGA39700.015.58175432
CCGCGTT165550.015.5579167
GTTCCTA64450.015.53142315
CGTACGC51050.015.4273455
CTCAGAC197500.015.3303131
CTACACG90700.015.32700445
CGATTAT49900.015.2868513
TCAGACC175050.015.1707232
CGCGTTC170200.015.1592948
ACGAACG41850.014.94250831
TTCACGC202350.014.8962427
Produced by FastQC (version 0.11.5)