FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45596544
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG560200.12286018870202092No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA525010.11514249851918602No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG493010.10812442276326908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG20700.013.91238226
GGGAGGT283900.013.1232283
AGCTTAG192400.012.50451845
ATAGGGG192050.012.49009538
AGGGATC166750.012.1970542
GGAGGTC314800.011.9494314
GGCATAG202600.011.70607235
TATCCTA115250.011.2830485
ATCCTAG116650.011.1664776
GTCACTC328800.011.1527258
GAGCTTA214050.011.14499144
TGTATCC115300.011.1027013
TAGGCTA107500.010.98697110
GGTCACT334500.010.982827
TGGCATA220400.010.92403634
CCTAGGC110050.010.8549978
TCCTAGG114600.010.7773387
CAGGGAT211050.010.6389211
GTTCTAT113700.010.54676151
CTAGGCT113150.010.49795159
Produced by FastQC (version 0.11.5)