FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43298265
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG122600.013.41567945
GGCATAG127950.012.48533735
GTTGTCG18500.012.28377626
GAGCTTA140300.011.78728944
ATAGGGG139050.011.71520238
TCAGACG83850.011.5921612
ATAGCTC66700.011.23312739
CATAGGG149700.010.83666637
ACCCGGC73600.010.7914335
TGGCATA151450.010.77087534
GGGAGGT227000.010.675143
GCATAGG156700.010.39565536
GGTCACT214900.010.155887
GTCACTC214450.010.1457158
ACTCTCC223400.09.9205411
ACGGACA82750.09.59818118
CAGACGT99800.09.5816913
TAGCTCT78750.09.57142340
CGTCGAT15350.09.5276812
GGAGGTC241500.09.4938164
Produced by FastQC (version 0.11.5)