FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47580504
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG871020.18306237361420133No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT484440.10181481053668537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCTT270500.019.7856462
TTCACGC260200.019.029877
TCACGCT264500.018.7204998
GCCGGCT246900.018.6318651
ACGCTCA279200.018.02493310
AACGCTA269900.017.78636723
ACGCTAC271600.017.67585624
AGACCGC145850.017.5224554
GACCGCG148400.017.1455635
CGCTACC281500.016.99876825
CGGCTTC333450.016.7925723
AAAACGC287700.016.73159421
ACCGCGT153250.016.6323076
CACGCTC299900.016.4732389
CAGACCG157250.016.4524383
AAACGCT298850.016.14621522
GAAAACG298650.016.12271520
GTCCGTT36700.015.6376181
CCGCGTT164400.015.5179497
CGCGTTC167950.015.2167328
Produced by FastQC (version 0.11.5)