FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47580504
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG729060.15322662408115728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT338450.014.8448483
GTTGTCG23200.014.353477526
GGAGGTC346050.014.2782524
AGCTTAG259200.014.21008245
ATAGGGG274600.013.51963438
GGCATAG278550.013.230988535
GAGCTTA294650.012.584434544
GGTCACT386400.012.5834217
TGGGAGG313300.012.4241672
TGGCATA301250.012.32362434
GTCACTC401250.012.1681858
CTGGGAG384500.011.1943711
ACTCTCC436700.011.1598711
CATAGGG342450.010.83441537
TCAGACG90400.010.702412
TCACTCT473750.010.614739
GCATAGG355700.010.4434936
CTCCCCA370700.010.415462514
GCTCTGT473750.010.32035922
AGGCTCT370700.010.2576320
Produced by FastQC (version 0.11.5)