FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45654109
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG779060.17064400490216555No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCA456670.1000282362317048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT279250.022.6983361
CCGGCTT316950.021.6882362
TCACGCT317600.020.9758388
TTCACGC324400.020.6263127
AACGCTA318150.020.5296623
ACGCTAC319800.020.40971224
CGCTACC325950.019.99736425
CGGCTTC359700.019.6735213
ACGCTCA346650.019.67234610
AAAACGC334850.019.59302521
GAAAACG343050.019.0983320
AAACGCT346000.018.97465922
CGTTACG19900.018.20275321
CACGCTC369300.018.1307189
CGTGGTT375550.017.37430241
GTCCGTT36250.016.3985231
TCGTGGT399200.016.3280840
CTACCTC417150.015.8630827
CGCTCAG435900.015.81994811
TACCTCT424750.015.51061828
Produced by FastQC (version 0.11.5)