FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45654109
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG605230.13256857121009633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG201850.014.99785645
ATAGGGG211650.014.33320938
GGGAGGT304050.014.2049263
GGCATAG212250.014.19703235
GTCACTC318700.013.2482148
GGTCACT317500.013.1848417
GGAGGTC330700.013.0326334
GAGCTTA234550.012.97396344
TGGCATA235850.012.92866934
ACTCTCC330300.012.77034411
AGGTCAC345650.012.4751256
CACCGAA216450.012.29123645
GAGGTCA360350.012.1349725
CACTCTC352000.011.95092910
GCACCGA225700.011.71757344
CATAGGG261200.011.67449137
TCACTCT365750.011.6424719
TGGGAGG293650.011.0540272
GCATAGG277300.011.02903736
GTTCTAT197950.011.0162771
Produced by FastQC (version 0.11.5)