FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45247602
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1091430.24121278294482879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG19450.020.0054576
AGACCGC257050.019.855034
CAGACCG263550.019.4848463
GAACGAT269450.018.9870945
GACCGCG278600.018.3272955
ACCGCGT285450.017.8861896
CCGCGTT300300.017.0091957
CGCGTTC300450.016.9707978
TCAGACC319550.016.4927962
CTCAGAC351250.016.2489991
CGTTACG16900.016.23733721
AGAACGA318750.016.11405444
GCGTTCT322050.015.8884679
CGGTATA32450.015.73440820
CCGTACG66650.015.2880344
GTCCGTT36300.015.1278391
CGTACGC67750.015.0398175
AAGAACG354800.014.49041143
ATACGGA358200.014.25182733
TACGGAG357900.014.23924234
Produced by FastQC (version 0.11.5)