FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45247602
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG503030.11117274236986084No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA462700.10225956283826931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG171650.013.8897145
ATAGGGG176800.013.45693938
GGGAGGT255650.013.1692643
GGCATAG181100.013.042006535
GAGCTTA199950.011.99432444
TGGCATA198850.011.86984234
GGTCACT273100.011.8488667
GTCACTC276800.011.7393878
GGAGGTC283650.011.6392614
ACTCTCC290650.011.30419311
GTTGTCG21800.011.15307426
CATAGGG217050.011.01457937
GCATAGG230850.010.3339836
GAGGTCA327700.010.3280565
CACTCTC316000.010.26940910
TCACTCT332750.010.0550179
TGGGAGG262900.09.7612532
GCTCTGT352900.09.22144822
TAGGGGA347850.09.19483439
AGGTCAC371650.09.1714786
Produced by FastQC (version 0.11.5)