FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46828369
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG16850.018.68996
ACGTCCG24900.017.7969232
GAACGAT102150.017.26458445
AGACCGC104850.016.8847794
CAGACCG111950.015.9746783
CCCGTAT28300.015.5706061
TCGTATG28450.015.41809645
CGTTACG14300.014.94558121
ACGTTCG19800.014.7692026
CCCGGAT157200.014.56458951
CGCAAAC43900.014.194591525
GTCCGTT38650.014.1630831
CGGATAC84050.014.0778753
ACGGTAC94250.013.74736745
CCCCCCT42350.013.7301971
TTCGGCG83750.013.72506744
CCGTACG83750.013.6716854
CGTACGC85150.013.4732625
TACGGTA96250.013.43833744
CCCGTCG48650.013.3995211
Produced by FastQC (version 0.11.5)