FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46828369
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA8800.016.6184537
ACCCGGC87100.014.7541835
GCGTCGT31050.013.47875211
TCGTGTA32600.013.18279914
TCACCCG102500.013.0220413
ATAGCTC99100.012.6484839
ATAGCGT11800.012.58394136
ACACGGA105400.012.20842416
CACCCGG110550.012.0125574
AGCTTAG102450.011.97288845
GTCGTGT36700.011.71021213
GTTGTCG18050.011.46545126
CGGACAG113450.011.26297519
CACGGAC120050.011.20579617
TAGCTCT112450.011.14706240
GGCATAG108650.011.11966935
TGTAGGG39850.010.89513717
GTAGGGA38700.010.75400118
TCAGACG81850.010.6425592
TGATAGC120300.010.54807837
Produced by FastQC (version 0.11.5)