FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44476667
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG132300.012.77203438
GGCATAG138500.012.08657135
AGCTTAG140900.012.05636645
GGGAGGT207250.011.9750963
GGTCACT211750.011.1889497
GTCACTC218550.011.05701358
GAGCTTA159400.010.72766644
ACTCTCC230350.010.55886911
GGAGGTC236850.010.49754
CATAGGG164150.010.2801737
TGGCATA164050.010.24529134
GCATAGG170550.09.97355636
CACTCTC257000.09.5865310
TCACTCT267050.09.5712499
GAGGTCA262500.09.5231655
TAGGCTA58350.09.29301510
AGGTCAC271450.09.1759996
TGGGAGG218700.08.4262272
GCTCTGT290650.08.34503922
TAGGGGA285150.08.2298339
Produced by FastQC (version 0.11.5)